منابع مشابه
Monocyte Nonspecific Esterase: Purification and Subunit Structure
Monocyte nonspecific esterase has been purified from cultured cells of the acute myeloid leukemia cell line. ML-l. The purified enzyme shows the characteristic properties of the monocyte neutral serine carboxyI esterase, with high sensitivity to organophosphorus inhibitors and sodium fluoride inhibitor. The enzyme is a membrane protein which in the native state exists as a monomer of a mol wt o...
متن کاملMonocyte nonspecific esterase: purification and subunit structure.
Monocyte nonspecific esterase has been purified from cultured cells of the acute myeloid leukemia cell line, ML-1. The purified enzyme shows the characteristic properties of the monocyte neutral serine carboxyl esterase, with high sensitivity to organophosphorus inhibitors and sodium fluoride inhibitor. The enzyme is a membrane protein which in the native state exists as a monomer of a mol wt o...
متن کاملDifferences in nonspecific esterase from normal and leukemic monocytes.
Nonspecific esterase (NSE) is used to identify normal and leukemic mononuclear phagocytes cytochemically. It can be demonstrated by the hydrolysis of alpha-naphthyl acetate or butyrate. NSE from leukapheresed leukemic monocytes were extracted with several types of detergent and grouped into two isoelectric point (pl) categories based on the ease of solubility: pl 5.7-6.3 (8 bands) greater than ...
متن کاملNonspecific esterase and E-rosette formation of bottle-nosed dolphin lymphocytes
N.N. Kavtsevich Murmansk marine biological institute KSC RAS, 17, Vladimirskaya str., Murmansk, 183010, Staining of bottle-nosed dolphin lymphocytes by nonspecific esterase stain and E-rosette test were performed. 35.4 % of lymphocytes were simultaneously E-rosetted and NE positive. They are apparently, mature T-cells. On the size and shape of esterase-positive granules at least 2 subpopulation...
متن کاملUltrastructural localization of nonspecific esterase activity in guinea pig and human monocytes, macrophages, and lymphocytes.
Using either hexazotized pararosaniline or new fuchsin as coupling agents, we investigated the ultrastructural localization of alpha-naphthyl acetate esterase (ANAE) activity in guinea pig bone marrow and peritoneal exudates, and in human peripheral blood cells. DFP-inhibitable ANAE activity was present on the cell surface of lymphocytes, monocytes, macrophages, neutrophils, eosinophils, basoph...
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ژورنال
عنوان ژورنال: Blood
سال: 1979
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v53.2.339.bloodjournal532339